Chapter 1 Introduction

A project to analyse the 16S bacterial profiles and to help determine storage protocols.

The dataset consist of 74 samples (out of 174) left after removal of poor quality and low read samples, and rarefaction size of 20k.

The samples were stored using different buffers. below is the number of samples by buffer method:

Ethanol: 12
No buffer: 16
PSP buffer: 16
RNAlater: 30

Samples were extracted from 6 patients. These patients had the following number of samples:

1 = 16
2 = 17
3 = 19
4 = 4
5 = 9
6 = 9